Probe the Interaction of Sodium Dodecyl Benzene Sulfonate to Papain on Molecular Level

Authors

  • Bin Zhang, Jin Guan, Dandan Guo and Rutao Liu School of Environmental Science and Engineering, Shandong University, China -America CRC for Environment and Health, Shandong Province, 27# Shanda South Road, Jinan 250100, P. R. China Author

Keywords:

Papain, SDBS, fluorescence spectra, synchronous fluorescence, UV-vis absorption spectroscopy.

Abstract

 In this paper, the effects of sodium dodecyl benzene sulfonate (SDBS) on the structure of papain were evaluated by the fluorescence spectroscopy and UV-vis absorption spectroscopy. The fluorescence emission spectra and synchronous fluorescence spectra indicated that there were interactions between SDBS and papain. Meanwhile, the microenvironment of the amino acid residues in the papain was changed. The UV-vis absorption results showed appreciable conformational changes in papain. The above results are significant in evaluating the toxic effects induced by SDBS in vivo

Published

2024-12-03

Issue

Vol. 3 No. 1 (2015)

Section

Articles